|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83127||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepEGFP-C1 with EGFP sequence removed
- Backbone size w/o insert (bp) 4242
- Total vector size (bp) 5874
Modifications to backboneEGFP sequence removed using Age I and BspE I
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemouse NFL
Alt nameneurofilament protein L
SpeciesM. musculus (mouse)
Insert Size (bp)1632
Entrez GeneNefl (a.k.a. AI847934, CMT2E, NF-L, NF68, Nfl)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.
Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmNFL was a gift from Anthony Brown (Addgene plasmid # 83127)
For your References section:The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons. Yan Y, Jensen K, Brown A. Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309. 10.1002/cm.20184 PubMed 17285620