pJ3 SHP2 E76A
(Plasmid #8321)

• Depositing Lab: Ben Neel
• Publication: Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Backbone

• Vector backbone: pJ3omega
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHP2
• Alt name: SH-PTP2
• Alt name: PTPN11
• Species: H. sapiens (human)
• Insert Size (bp): 2276
• Mutation: E76A (activated mutant)
• GenBank ID: L03535
• Entrez Gene: PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The SHP2 E76A mutation is described in "Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps" O'Reilly AM, Pluskey S, Shoelson SE, Neel BG. Mol Cell Biol. 2000 Jan;20(1):299-311. PubMed ID: 10594032