pAd388-shLuc
(Plasmid
#83275)
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PurposeshRNA targeting luciferace
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Login to view industry pricing.
Backbone
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Vector backbonepAd388
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Backbone manufacturerOD260 Inc
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA to Luciferase
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gRNA/shRNA sequenceLuciferase
- Promoter pUC ori
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer none (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAd388-shLuc was a gift from Joe Landry (Addgene plasmid # 83275 ; http://n2t.net/addgene:83275 ; RRID:Addgene_83275) -
For your References section:
BPTF inhibits NK cell activity and the abundance of natural cytotoxicity receptor co-ligands. Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic B, Dumur CI, Landry JW. Oncotarget. 2017 May 12;8(38):64344-64357. doi: 10.18632/oncotarget.17834. eCollection 2017 Sep 8. 10.18632/oncotarget.17834 PubMed 28969075
