Addgene - pgex-cIAP1 Plasmid Data

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Plasmid 8328: pgex-cIAP1

Gene/insert name:
cIAP1
Insert size:
1700
Species:
H. sapiens (human)
Entrez Gene:
BIRC2 (API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX
(Search Vector Database)
Backbone manufacturer:
amersham
Vector type:
Bacterial Expression
Backbone size w/o insert:
4900
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
GGGCTGGCAAGCCACGTTTGGTGG List of Sequencing Primers
3' sequencing primer:
TCCGGGAGCTGCATGTGTCAGAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jon Ashwell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li et al (Nature 2002 Mar 21;416(6878):345-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8328" in your Materials and Methods section.