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pdCas9-Tet1-CD
(Plasmid #83340)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83340 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX330
  • Backbone manufacturer
    Received from Feng Zhang (MIT)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
  • Species
    Synthetic
  • Mutation
    D10A, H840A
  • Promoter U6, CBH

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdCas9-Tet1-CD was a gift from Ronggui Hu (Addgene plasmid # 83340 ; http://n2t.net/addgene:83340 ; RRID:Addgene_83340)
  • For your References section:

    A CRISPR-based approach for targeted DNA demethylation. Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu GL, Hu R. Cell Discov. 2016 May 3;2:16009. doi: 10.1038/celldisc.2016.9. eCollection 2016. 10.1038/celldisc.2016.9 PubMed 27462456