pET28b(+)-SPN+IFS
(Plasmid #83372)

• Purpose: Expresses SPN and IFS complex in E. coli cell
• Depositing Lab: SeWon Suh
• Publication: Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):962-7. doi: 10.1107/S0909049513020803. Epub 2013 Sep 29.

Backbone

• Vector backbone: pET28b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 7099
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: SPN
• Alt name: Nicotine adenine dinucleotide glycohydrolase
• Alt name: Nga
• Species: Streptococcus pyogenes serotype M3
• Insert Size (bp): 1245
• GenBank ID: AAM78735.1
• Promoter: T7
• Tag / Fusion Protein:
  • Hexahistidine tag (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: None (not destroyed)
• 5′ sequencing primer: T7 promoter

Gene/Insert 2

• Gene/Insert name: IFS
• Species: Streptococcus pyogenes serotype M3
• Insert Size (bp): 486
• GenBank ID: AAM78736.1
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry