Addgene - pcdna3.1 hciap2 Plasmid Data

Plasmid 8338: pcdna3.1 hciap2

Gene/insert name:
cIAP2
Insert size:
1800
Species:
H. sapiens (human)
Entrez Gene:
BIRC3 (AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2)
Fusion protein or tag:
myc
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
Xho1
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (5)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Jon Ashwell
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

There is a N100D change in the amino acid sequence and, according to the author, this mutation does not affect protein function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8338" in your Materials and Methods section.