h-gamma-ENaC Myc
(Plasmid #83428)

• Purpose: full length human gamma ENaC with myc tag
• Depositing Lab: Christie Thomas
• Publication: Raikwar et al Am J Physiol Renal Physiol. 2008 May;294(5):F1157-65. doi: 10.1152/ajprenal.00339.2007. Epub 2008 Mar 5.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: thermofisher
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human gamma ENaC
• Species: H. sapiens (human)
• Insert Size (bp): 2045
• Mutation: P502A
• Entrez Gene: SCNN1G (a.k.a. BESC3, ENaCg, ENaCgamma, LDLS2, PHA1, PHA1B3, SCNEG)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note the ENaC insert in this plasmid contains a single nucleotide mismatch compared to Genbank reference sequence AAA75460.1, which causes a P502A mutation of unknown significance. The ENaC sequence containing 502A was cloned and reported in McDonald et al., Am J Physiol. 1995 May; 268(5 Pt 1): C1157-63. PMID: 7762608 (see Figure 1B) and was the source of the ENaC insert for this plasmid.