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Purposefull length human beta ENaC with V5 tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerthermofisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman beta ENaC
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SpeciesH. sapiens (human)
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Insert Size (bp)2075
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Entrez GeneSCNN1B (a.k.a. BESC1, ENaCb, ENaCbeta, LIDLS1, PHA1B2, SCNEB, beta-ENaC, beta-NaCH)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
h-beta-ENaC was a gift from Christie Thomas (Addgene plasmid # 83429 ; http://n2t.net/addgene:83429 ; RRID:Addgene_83429) -
For your References section:
Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel. Raikwar NS, Thomas CP. Am J Physiol Renal Physiol. 2008 May;294(5):F1157-65. doi: 10.1152/ajprenal.00339.2007. Epub 2008 Mar 5. 10.1152/ajprenal.00339.2007 PubMed 18322022
