hKDR
(Plasmid #83436)

• Purpose: human VEGFR2 with V5 and tGFP tag
• Depositing Lab: Christie Thomas
• Publication: Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;315(2):C214-C224. doi: 10.1152/ajpcell.00247.2017. Epub 2018 May 2.

Backbone

• Vector backbone: pCMV6 Entry
• Backbone manufacturer: Origene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human VEGFR2
• Species: H. sapiens (human)
• Insert Size (bp): 4982
• Entrez Gene: KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 (N terminal on insert)
  • turboGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also see Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi: 10.1371/journal.pone.0112794. eCollection 2014.