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pML104-KanMx4
(Plasmid #83476)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83476 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSII426
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB dam-/dcm-
  • Growth instructions
    Growth in dam- E.coli cells is preferred
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KanMX4

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #67638 pML104, deposited by John Wyrick
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is similar to pML104 except that the URA3 marker has been switched for KanMX4 marker using Gibson Assembly. Cloning of guide sequence using BclI-SwaII restriction sites requires growth in dam- E. coli cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML104-KanMx4 was a gift from Patricia Wittkopp (Addgene plasmid # 83476 ; http://n2t.net/addgene:83476 ; RRID:Addgene_83476)