pcDL-hu FceRI alpha
(Plasmid #8365)

• Depositing Lab: Jean Kinet
• Publication: Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584.

Backbone

• Vector backbone: pcDL SR alpha 296
• Backbone manufacturer: K Arai DNAX
• Backbone size w/o insert (bp): 3741
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Fc epsilon RI alpha cDNA
• Alt name: high affinity receptor for IgE alpha chain
• Species: H. sapiens (human)
• Insert Size (bp): 1177
• GenBank ID: X06948
• Entrez Gene: FCER1A (a.k.a. FCE1A, FCERIA, FcERI)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GTT GCC TTT ACT TCT AGG CCT
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For information about the empty vector, see Yokota et al - PNAS Nov 1987. Vol 84, p 7388-7392.