pGEM-hu FceRI beta
(Plasmid
#8366)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8366 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM 3Zf-
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3199
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Fc epsilon RI beta cDNA
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Alt namehigh affinity receptor for IgE beta chain
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Alt namemembrane-spanning 4 domains
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SpeciesH. sapiens (human)
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Insert Size (bp)760
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GenBank IDM89796
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Entrez GeneMS4A2 (a.k.a. APY, ATOPY, FCER1B, FCERI, IGEL, IGER, IGHER, MS4A1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-hu FceRI beta was a gift from Jean Kinet (Addgene plasmid # 8366 ; http://n2t.net/addgene:8366 ; RRID:Addgene_8366) -
For your References section:
The gene and cDNA for the human high affinity immunoglobulin E receptor beta chain and expression of the complete human receptor. Kuster H, Zhang L, Brini AT, MacGlashan DW, Kinet JP. J Biol Chem 1992 Jun 25;267(18):12782-7. PubMed 1535625