2a-copGFP(+HA)
(Plasmid #83806)

• Purpose: Express copGFP reporter for HDR-induced knock-in
• Depositing Lab: Bo Feng
• Publication: He et al Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4.

Backbone

• Vector backbone: psuper puro
• Backbone manufacturer: 4353
• Total vector size (bp): 5648
• Modifications to backbone: Primers carrying Picornavirus “self-cleaving” P2a sequence and cloning sites were synthesized and used for amplifying the DNA fragment carrying P2a-copGFP joint-coding sequence from plasmid PCDH-CMV-MCS-EF1-copGFP (System Biosciences). The fragment obtained was inserted into BamHI and XhoI sites in pSuper-puro vector. Two homology arms were amplified from GAPDH genomic locus, and inserted into MfeI and MluI sites at 5’ and HpaI and XhoI sites at 3’ of the 2a-copGFP fragment.
• Vector type: Homologous recombination for GAPDH loci

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: copGFP

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry