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2a-copGFP(+HA)
(Plasmid #83806)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83806 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    psuper puro
  • Backbone manufacturer
    4353
  • Total vector size (bp) 5648
  • Modifications to backbone
    Primers carrying Picornavirus “self-cleaving” P2a sequence and cloning sites were synthesized and used for amplifying the DNA fragment carrying P2a-copGFP joint-coding sequence from plasmid PCDH-CMV-MCS-EF1-copGFP (System Biosciences). The fragment obtained was inserted into BamHI and XhoI sites in pSuper-puro vector. Two homology arms were amplified from GAPDH genomic locus, and inserted into MfeI and MluI sites at 5’ and HpaI and XhoI sites at 3’ of the 2a-copGFP fragment.
  • Vector type
    Homologous recombination for GAPDH loci

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    copGFP

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2a-copGFP(+HA) was a gift from Bo Feng (Addgene plasmid # 83806 ; http://n2t.net/addgene:83806 ; RRID:Addgene_83806)
  • For your References section:

    Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, Feng B. Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. Epub 2016 Feb 4. 10.1093/nar/gkw064 PubMed 26850641