Addgene - pCMV-SHP2 CS Plasmid Data

Plasmid 8382: pCMV-SHP2 CS

Gene/insert name:
SHP2
Alt name:
SH-PTP2
Alt name:
PTPN11
Alt name:
PTP2C
Insert size:
2276
Species:
H. sapiens (human)
Entrez Gene:
PTPN11 (BPTP3, CFC, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
C459S, mutation of essential catalytic cysteine
Vector backbone:
pRC/CMV
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5542
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Ben Neel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8382" in your Materials and Methods section.