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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
(Plasmid #83925)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83925 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW
  • Total vector size (bp) 15000
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    717
  • GenBank ID
    FJ226077.1
  • Promoter hUbC
  • Tag / Fusion Protein
    • P2A (C terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Bsr
  • Alt name
    blasticidin-S deaminase from Bacillus cereus
  • Insert Size (bp)
    422
  • Tag / Fusion Protein
    • P2A (N terminal on insert)

Resource Information

Depositor Comments

Digest with BsmBI to remove ~1.9 kb stuffer for cloning in protospacer sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-U6-gRNA-UbC-eGFP-P2A-Bsr was a gift from Charles Gersbach (Addgene plasmid # 83925)
  • For your References section:

    CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. 10.1038/nbt.3853 PubMed 28369033