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PurposeFRET donor for dynamically measuring TrkB activity in neurons
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTropomyosin related kinase B
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Alt nameTrkB
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SpeciesM. musculus (mouse)
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Insert Size (bp)3204
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Entrez GeneNtrk2 (a.k.a. GP145-TrkB/GP95-TrkB, Tkrb, trk-B, trkB)
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TrkB mEGFP was a gift from Ryohei Yasuda (Addgene plasmid # 83952 ; http://n2t.net/addgene:83952 ; RRID:Addgene_83952) -
For your References section:
Autocrine BDNF-TrkB signalling within a single dendritic spine. Harward SC, Hedrick NG, Hall CE, Parra-Bueno P, Milner TA, Pan E, Laviv T, Hempstead BL, Yasuda R, McNamara JO. Nature. 2016 Sep 28;538(7623):99-103. doi: 10.1038/nature19766. 10.1038/nature19766 PubMed 27680698
