Addgene - pMTH PKC epsilon Plasmid Data

Plasmid 8413: pMTH PKC epsilon

Gene/insert name:
Mouse Protein Kinase C epsilon
Alt name:
PKC epsilon
Alt name:
PKCe
Alt name:
Protein Kinase C epsilon
Insert size:
2400
Species:
M. musculus (mouse)
Entrez Gene:
Prkce (5830406C15Rik, PKC[e], PKCepsilon, Pkce, R75156)
Vector backbone:
pMTH
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
11500
Cloning site 5':
BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
The pMTH backbone was a gift from Dr. Michael Kuehl (backbone map uploaded here).
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Frederic Mushinski
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. Mischak et al (J Biol Chem 1993 Sep 25;268(27):20110-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8413" in your Materials and Methods section.