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pMIA14
(Plasmid #8424)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8424 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    intracisternal A-particle gene
  • Alt name
    IAP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    13000
  • GenBank ID
    M17551
  • Entrez Gene
    Iap (a.k.a. ERV class II)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIA14 was a gift from Dean Hamer (Addgene plasmid # 8424)

  • For your References section:

    Nucleotide sequence of a complete mouse intracisternal A-particle genome: relationship to known aspects of particle assembly and function. Mietz JA, Grossman Z, Lueders KK, Kuff EL. J Virol 1987 Oct;61(10):3020-9. PubMed 3041022
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