pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP
PurposeExpresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84256||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7646
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameSa sgCXCR4-3
SpeciesH. sapiens (human), Synthetic; S. aureus
- Promoter mouse U6
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GAGATCCAGTTTGGTTAGTACCGGG
- 3′ sequencing primer ATGCATGGCGGTAATACGGTTAT (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe original Sa sgRNA scaffold was a gift from Feng Zhang (MIT) and was modified based on a design by Bo Huang (UCSF).
Terms and Licenses
Contains a CMV-EGFP fluorescent marker 3' of the sgRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP was a gift from Stanley Qi (Addgene plasmid # 84256 ; http://n2t.net/addgene:84256 ; RRID:Addgene_84256)
For your References section:Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. 10.1038/nmeth.4042 PubMed 27776111