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Purpose(Empty Backbone) A versatile vector for recombinant mycobacterial protein production
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAL5000, pUC19
- Backbone size (bp) 5994
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Modifications to backboneInsertion of the gene AmiC and AmiA of M. smegmatis
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Vector typeBacterial Expression
- Promoter Aceteamidase
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsit is a shuttle vector, to be used in Mycobacterium smegmatis
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Copy numberLow Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMyCA was a gift from Young-Hwa Song (Addgene plasmid # 84268 ; http://n2t.net/addgene:84268 ; RRID:Addgene_84268) -
For your References section:
A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon. Magana Vergara C, Kallenberg CJL, Rogasch M, Hubner CG, Song YH. Protein Sci. 2017 Aug 31. doi: 10.1002/pro.3288. 10.1002/pro.3288 PubMed 28857325
Map uploaded by the depositor.
