pUC19 PKC alpha
(Plasmid #8429)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Mouse Protein Kinase C alpha
  • Alt name
    Protein Kinase C alpha
  • Alt name
    PKC alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    M25811
  • Entrez Gene
    Prkca (a.k.a. RP23-145G23.2, AI875142, Pkca)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 Forward20
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stefan Rose-John
  • Terms and Licenses

Depositor Comments

Please cite "Gene. 1988 Dec 30;74(2):465-71" if you use this plasmid in your publication.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19 PKC alpha was a gift from Frederic Mushinski (Addgene plasmid # 8429)