pAd-Track SIRT1 H355A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8441||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9220
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2200
MutationHistidine 355 mutated to Alanine
Entrez GeneSirt1 (a.k.a. AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer No (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Track SIRT1 H355A was a gift from Pere Puigserver (Addgene plasmid # 8441)
For your References section:Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P. Nature 2005 Mar 3;434(7029):113-8. 10.1038/nature03354 PubMed 15744310
Generated by Addgene from plasmid data.