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pRK5-Flag CASTOR1 ACT1
(Plasmid #84491)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84491 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CASTOR1 ACT1
  • Species
    H. sapiens (human)
  • Mutation
    1-169 amino acid
  • Entrez Gene
    GATSL3 (a.k.a. CASTOR1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer TGT AAC CAT TAT AAG CTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-Flag CASTOR1 ACT1 was a gift from David Sabatini (Addgene plasmid # 84491)
  • For your References section:

    The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, Wang T, Harper JW, Gygi SP, Sabatini DM. Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. Epub 2016 Mar 10. 10.1016/j.cell.2016.02.035 PubMed 26972053