Addgene - Lentihair s1GFP Plasmid Data

Plasmid 8451: Lentihair s1GFP

Gene/insert name:
siRNA against GFP
Vector backbone:
pLentihair
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Cloning site 5':
ApaI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Weinberg-viral (application/pdf)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Lentiviral vector for siRNA expression, see also pLKO.1. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8451" in your Materials and Methods section.