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pCIneoRL-BCL2 3'UTR-mir200c-mut
(Plasmid #84598)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 84598 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL2 3'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • Mutation
    Mutation on miR-200c binding site on BCL2 3'UTR
  • Promoter CMV
  • Tag / Fusion Protein
    • Renilla Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer RenLuc3F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneoRL-BCL2 3'UTR-mir200c-mut was a gift from Yanan Yang (Addgene plasmid # 84598 ; http://n2t.net/addgene:84598 ; RRID:Addgene_84598)
  • For your References section:

    A genetic cell context-dependent role for ZEB1 in lung cancer. Zhang T, Guo L, Creighton CJ, Lu Q, Gibbons DL, Yi ES, Deng B, Molina JR, Sun Z, Yang P, Yang Y. Nat Commun. 2016 Jul 26;7:12231. doi: 10.1038/ncomms12231. 10.1038/ncomms12231 PubMed 27456471