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pMyBADNT-kan
(Plasmid #84691)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84691 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMyNT-kan
  • Backbone manufacturer
    Wilmanns lab
  • Backbone size (bp) 6832
  • Modifications to backbone
    The acetamidase-inducible promoter in pMyNT-kan was replaced by the E. coli arabinose-inducible promoter from pBADM11 vector using Gibson cloning. The pMyNT-kan backbone was amplified using the primers: 5’-caccaccaccaccaccacccgtcg and 5’-atttaaagatctggtaccgcggcc. The arabinose-inducible promoter from the pBAD vector was amplified using the primers: 5’-acaggttctcgccggccgacgggtggtggtggtggtggtgcatggttaattcctcctgttagc and 5’-gtggcatccgtggcgcggccgcggtaccagatctttaaatctagattatgac.
  • Vector type
    Bacterial Expression
  • Promoter AraC
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCTGACGCTTTTTATCGCAACTC
  • 3′ sequencing primer TTGATGACGAGCGTAATGGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyBADNT-kan was a gift from Matthias Wilmanns (Addgene plasmid # 84691 ; http://n2t.net/addgene:84691 ; RRID:Addgene_84691)
  • For your References section:

    The pMy vector series: A versatile cloning platform for the recombinant production of mycobacterial proteins in Mycobacterium smegmatis. Beckham KSH, Staack S, Wilmanns M, Parret AHA. Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962. 10.1002/pro.3962 PubMed 33006405