|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84781||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5314
- Total vector size (bp) 5334
Vector typeMammalian Expression, Bacterial Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameminor satellite-specific sgRNA
- Promoter U6
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer 5´- GAG GGC CTA TTT CCC ATG ATT C-3´ (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEX-A-U6-MiSgRNA_PuroR was a gift from Heinrich Leonhardt (Addgene plasmid # 84781 ; http://n2t.net/addgene:84781 ; RRID:Addgene_84781)
For your References section:Determination of Local Chromatin Composition by CasID. Schmidtmann E, Anton T, Rombaut P, Herzog F, Leonhardt H. Nucleus. 2016 Sep 27:0. 10.1080/19491034.2016.1239000 PubMed 27676121