Addgene - pET-UbcH10-DN Plasmid Data

Plasmid 8502: pET-UbcH10-DN

Gene/insert name:
UbcH10
Alt name:
ubiquitin-conjugating enzyme H10
Insert size:
540
Species:
H. sapiens (human)
Entrez Gene:
UBE2C (RP3-447F3.1, UBCH10, dJ447F3.2)
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Mutation:
Dominant Negative, C114S
Vector backbone:
peT29a
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5242
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 Forward List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Joan Ruderman
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

See author's map. For bacterial expression of C-terminal His-tagged UbcH10-wt. (Ruderman pET29-wt)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cell cycle-regulated proteolysis of mitotic target proteins. Bastians et al (Mol Biol Cell 1999 Nov;10(11):3927-41. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8502" in your Materials and Methods section.