pPdX
(Plasmid #85084)

• Purpose: Expresses putidaredoxin in E. coli
• Depositing Lab: Teruyuki Nagamune
• Publication: Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.

Backbone

• Vector backbone: pET-28b(+)
• Backbone manufacturer: Merck Millipore
• Backbone size w/o insert (bp): 5333
• Total vector size (bp): 5657
• Modifications to backbone: N/A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Putidaredoxin
• Alt name: PdX
• Species: Synthetic; Pseudomonas putida
• Insert Size (bp): 324
• Mutation: changed Cysteine 73 to Serine and Cysteine 85 to Serine
• GenBank ID: BAA00414.1
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His6 tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PdX gene was subcloned from a synthetic gene whose codons are optimized for E. coli expression.