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Purposeexogenous AR expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLENTI6.3/V5-DEST
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameandrogen receptor
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Alt nameAR
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SpeciesH. sapiens (human)
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GenBank IDNM_000044
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer V5-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLENTI6.3/AR-GC-E2325 was a gift from Karl-Henning Kalland (Addgene plasmid # 85128 ; http://n2t.net/addgene:85128 ; RRID:Addgene_85128) -
For your References section:
Context dependent regulatory patterns of the androgen receptor and androgen receptor target genes. Olsen JR, Azeem W, Hellem MR, Marvyin K, Hua Y, Qu Y, Li L, Lin B, Ke X, Oyan AM, Kalland K. BMC Cancer. 2016 Jul 4;16:377. doi: 10.1186/s12885-016-2453-4. 10.1186/s12885-016-2453-4 [pii] PubMed 27378372
