HOXB6 (human) FLAG pMSCV
(Plasmid #8534)

• Depositing Lab: Corey Largman
• Publication: Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40.

Backbone

• Vector backbone: MSCV
• Backbone size w/o insert (bp): 6400
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP for FACS sorting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXB6
• Alt name: HOX2B
• Alt name: HOX2.2
• Species: H. sapiens (human)
• Insert Size (bp): 771
• Mutation: contains improved ribosomal binding site and FLAG epitope tag on N-terminal, starts at AA; #2, makes protein by western; contains GFP driven from an IRES
• GenBank ID: X58431 BC014651
• Entrez Gene: HOXB6 (a.k.a. HOX2, HOX2B, HU-2, Hox-2.2)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • IRES-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI/HpaI blunt (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map.