HOXB8 (murine) HIS-tag pET
(Plasmid #8540)

• Depositing Lab: Corey Largman
• Publication: Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-906.

Backbone

• Vector backbone: pET28b
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXB8
• Alt name: HOX2D
• Alt name: HOX2.4
• Species: M. musculus (mouse)
• Insert Size (bp): 828
• Mutation: starts at amino acid #2 (Ser)
• GenBank ID: X137221
• Entrez Gene: Hoxb8 (a.k.a. Hox-2.4)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jerry Adams

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Kongsuwan, K., Allen, J., Adams, J. M.Nuc. Acids Res. 17: 1881-1892, 1989