pMXs-E55A-ATPIF1
(Plasmid #85404)

• Purpose: Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
• Depositing Lab: David Sabatini
• Publication: Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 10.1016/j.celrep.2014.02.046. Epub 2014 Mar 27.

Backbone

• Vector backbone: pMXs rtv-016
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATPIF1
• Species: H. sapiens (human)
• Mutation: E55A point mutation abrogating the ability of ATPIF1 to bind the ATP synthase
• Entrez Gene: ATP5IF1 (a.k.a. ATPI, ATPIF1, ATPIP, IP)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AGTAGACGGCATCGCAGCTTGGATA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry