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pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
(Plasmid #85453)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85453 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO
  • Total vector size (bp) 13840
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-sgRNA-Backbone-UP-TdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    6913
  • Mutation
    deletion of AA123-365 in tdTomato (please see depositor comment below)
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that deletion of AA123-365 in tdTomato was found during Addgene's quality control. The depositor noted that this deletion does NOT affect plasmid function.

Any issue or question regarding this vector can be also directed to Le Cong (Regev lab) at [email protected].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE was a gift from Aviv Regev (Addgene plasmid # 85453 ; http://n2t.net/addgene:85453 ; RRID:Addgene_85453)
  • For your References section:

    A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells. Singer M, Wang C, Cong L, Marjanovic ND, Kowalczyk MS, Zhang H, Nyman J, Sakuishi K, Kurtulus S, Gennert D, Xia J, Kwon JY, Nevin J, Herbst RH, Yanai I, Rozenblatt-Rosen O, Kuchroo VK, Regev A, Anderson AC. Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052. 10.1016/j.cell.2016.08.052 PubMed 27610572