pAc-GFP-dSUMOdeltaC
(Plasmid #85697)

• Purpose: expression of GFP-SUMO1 delta C in Drosophila cells
• Depositing Labs: Licio Collavin, Giannino Del Sal
• Publication: Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-56.

Backbone

• Vector backbone: pAc5.1/V5 HIS A
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5400
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SUMO
• Alt name: CG4494 gene product from transcript CG4494-RA
• Alt name: SUMO-1
• Alt name: dSUMO
• Species: D. melanogaster (fly)
• Mutation: non-conjugatable SUMO, lacking the C-terminal glycines necessary for attachment to substrates
• Entrez Gene: Sumo (a.k.a. Dmel_CG4494, CG4494, Dm0342, DmSUMO-1, DmSmt3, Dmel\CG4494, Dmsmt3, SMT3, SUMO, Smt3, anon-EST:Posey240, dSUMO, dSmt3, dsmt3, l(2)04493, l(2)SH0182, l(2)SH2 0182, smt3, sumo)
• Promoter: pAc5
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: AC5
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry