Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85700||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRpR 150 enhancer and hsp70 minimal promoter
SpeciesD. melanogaster (fly)
- Promoter RpR 150 enhancer and hsp70 minimal promoter
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRpr150-LUC was a gift from Licio Collavin & Giannino Del Sal (Addgene plasmid # 85700 ; http://n2t.net/addgene:85700 ; RRID:Addgene_85700)
For your References section:Modification of Drosophila p53 by SUMO modulates its transactivation and pro-apoptotic functions. Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky MH, Collavin L. J Biol Chem. 2008 Jul 25. 283(30):20848-56. 10.1074/jbc.M710186200 PubMed 18492669