pQE60-mPU.1-ETS
(Plasmid #85733)

• Purpose: Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4
• Depositing Lab: Gregory Poon
• Publication: Poon et al J Biol Chem. 2012 May 25;287(22):18297-307. doi: 10.1074/jbc.M112.342345. Epub 2012 Apr 2.

Backbone

• Vector backbone: pQE-60
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3431
• Total vector size (bp): 3700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): M15
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ETS domain of transcription factor PU.1
• Alt name: Spi1
• Species: M. musculus (mouse)
• Insert Size (bp): 363
• GenBank ID: M38252.1 AAA40110.1
• Entrez Gene: Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
• Tag / Fusion Protein:
  • 6xHis tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: GTTCTGAGGTCATTACTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

M15 cells were purchased from Qiagen already pre-transformed with a plasmid pREP-4, which expresses the lac repressor. pREP-4 confers additional kanamycin resistance.