pX330-sgAAVS1
(Plasmid #85802)

• Purpose: Cas9/CRISPR vector for cut at human AAVS1 gene intron1
• Depositing Lab: Roland Friedel
• Publication: Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 10.1016/j.ebiom.2019.03.064.

Backbone

• Vector backbone: pX330
• Backbone manufacturer: Feng Zhang laboratory (Addgene Plasmid #42230 )
• Backbone size w/o insert (bp): 8488
• Total vector size (bp): 8508
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPP1R12C
• Alt name: AAVS1 insertion site (gene symbol PPP1R12C)
• gRNA/shRNA sequence: GGGGCCACTAGGGACAGGAT
• Species: H. sapiens (human)
• Entrez Gene: PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: U6-fwd

Resource Information

• Supplemental Documents:
  • Annotation-pX330-sgAAVS1-Addgene85802

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry