Addgene - pBABE-neo largeTcDNA K1 Plasmid Data

Plasmid 8581: pBABE-neo largeTcDNA K1

Gene/insert name:
large T antigen
Alt name:
large T cDNA
Alt name:
SV40gp6
Alt name:
LTc
Insert size:
2200
Species:
simian virus
Entrez Gene:
SV40gp6 (SV40gp6)
Mutation:
E107K. Alteration in pRB consensus binding motif (LxCxE). No longer binds to pRB family members.
Vector backbone:
pBABE-neo
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5330
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn et al (Mol Cell Biol 2002 Apr;22(7):2111-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8581" in your Materials and Methods section.