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pGEX-2T SHP1 WT
(Plasmid #8594)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8594 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHP1
  • Alt name
    SH-PTP1
  • Alt name
    PTPN6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    PTPN6 (a.k.a. HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linker between GST and SHP1 13aa of 5' UTR was used. (leu-his-ile-phe-ser-glu-asn-pro-arg-met of SHP1)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T SHP1 WT was a gift from Ben Neel (Addgene plasmid # 8594 ; http://n2t.net/addgene:8594 ; RRID:Addgene_8594)
  • For your References section:

    Isolation of a src homology 2-containing tyrosine phosphatase. Plutzky J, Neel BG, Rosenberg RD. Proc Natl Acad Sci U S A 1992 Feb 1;89(3):1123-7. 10.1073/pnas.89.3.1123 PubMed 1736296