-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 8601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepJ3H
- Backbone size w/o insert (bp) 3500
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePTP-1B
-
Alt namePTPN1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1400
-
MutationK103R
-
Entrez GenePTPN1 (a.k.a. PTP1B)
-
Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byJ. Chernoff, Fox Chase Cancer Center
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJ3H PTP-1B was a gift from Ben Neel (Addgene plasmid # 8601 ; http://n2t.net/addgene:8601 ; RRID:Addgene_8601)