pMT416
(Plasmid #8607)

• Depositing Lab: Robert Hartley
• Publication: Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Backbone

• Vector backbone: partial pUC19
• Backbone size w/o insert (bp): 1817
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Requires strain with the lacI^Q gene (such as DH5alpha, XL1-blue, SURE and HB107)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: barnase, barstar
• Species: Bacillus amyloliquefaciens
• Insert Size (bp): 1083
• GenBank ID: M14442 X15545
• Tag / Fusion Protein:
  • phoA signal sequence to barnase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Barnase production plasmid. Same as pMT413 except vector is a reversed subsequence of pUC. Requires an E. coli host carrying the lacI^Q gene.