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pMT3017
(Plasmid #8611)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8611 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    derivative of pUC19
  • Backbone size w/o insert (bp) 2440
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    barstar
  • Species
    Bacillus amyloliquefaciens
  • Insert Size (bp)
    439
  • Mutation
    A silent mutation in the chloramphenicol resistance gene has removed an EcoR1 site.
  • GenBank ID
    X15545

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As pMT316 but with the ampicillin resistance gene replaced by the chloramphenicol gene from plasmid pACYC184. A silent mutation in the CmR gene has removed an EcoR1 site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT3017 was a gift from Robert Hartley (Addgene plasmid # 8611)

  • For your References section:

    Barnase-barstar interaction. Hartley RW. Methods Enzymol 2001;341:599-611. 10.1016/S0076-6879(01)41179-7 PubMed 11582808
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