PurposeRetroviral vector expressing RAC1(G12V)-IRES-Bsd-p2A-RFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86139||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
- Promoter LTR
/ Fusion Protein
- 3xFLAG (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer TACACGCCGCCCACGTGAAG (Common Sequencing Primers)
NOTE: GFP sequence present after the stop codon of the ORF that is not translated. RAC1 sequence is codon-optimized.
Addgene's sequencing results indicate a 3 base ambiguity in the blasticidin resistance gene. It is unclear if this sequence variant affects blasticidin resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs2-RAC1 G12V was a gift from David Sabatini (Addgene plasmid # 86139 ; http://n2t.net/addgene:86139 ; RRID:Addgene_86139)
For your References section:Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM. Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. 10.1016/j.cell.2017.01.013 PubMed 28162770