pstb-LAFR5-exoY
(Plasmid
#86170)
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Purposepstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepstb-LAFR5
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Backbone manufacturerKay Jones
- Total vector size (bp) 22700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameexoY
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SpeciesS. meliloti
- Promoter Smb21651
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pstb-LAFR5-exoY was a gift from Kay Jones (Addgene plasmid # 86170 ; http://n2t.net/addgene:86170 ; RRID:Addgene_86170) -
For your References section:
Increased production of the exopolysaccharide succinoglycan enhances Sinorhizobium meliloti 1021 symbiosis with the host plant Medicago truncatula. Jones KM. J Bacteriol. 2012 Aug;194(16):4322-31. doi: 10.1128/JB.00751-12. Epub 2012 Jun 8. 10.1128/JB.00751-12 PubMed 22685282
