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Human LFA-1 alpha
(Plasmid #8630)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8630 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAPRM8
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LFA1 alpha
  • Alt name
    CD11a
  • Alt name
    pCDL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4322
  • GenBank ID
    NM_002209
  • Entrez Gene
    ITGAL (a.k.a. CD11A, LFA-1, LFA1A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human LFA-1 alpha was a gift from Timothy Springer (Addgene plasmid # 8630 ; http://n2t.net/addgene:8630 ; RRID:Addgene_8630)
  • For your References section:

    Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. Larson RS, Corbi AL, Berman L, Springer T. J Cell Biol 1989 Feb;108(2):703-12. 10.1083/jcb.108.2.703 PubMed 2537322