Human MAC-1 alpha
(Plasmid #8631)

• Depositing Lab: Timothy Springer
• Publication: Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

Backbone

• Vector backbone: pCDM8
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4356
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061/P3
• Growth instructions: The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAC-1 alpha
• Alt name: pCDM1
• Alt name: CD11b
• Species: H. sapiens (human)
• Insert Size (bp): 4200
• GenBank ID: BC096348
• Entrez Gene: ITGAM (a.k.a. CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstXI (not destroyed)
• 3′ cloning site: BstXI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This clone will direct the expression of functional Mac-1 upon cotransfection with the beta2 subunit in cos cells. This sequence has been deposited in Genbank with accession number J03925. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).