Purpose(Empty Backbone) A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86461||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4500
Vector typeMammalian Expression, Luciferase
- Promoter MYC
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer RVprimer3 CTAGCAAAATAGGCTGTCCC
- 3′ sequencing primer L4440 AGCGAGTCAGTGAGCGAG (Common Sequencing Primers)
Use FspI and AclI to remove the kanamycin resistance cassette and replace with putative regulatory elements by Gibson cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL4.23-MYC was a gift from Eric Lander (Addgene plasmid # 86461 ; http://n2t.net/addgene:86461 ; RRID:Addgene_86461)
For your References section:Systematic mapping of functional enhancer-promoter connections with CRISPR interference. Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, Kane M, Cleary B, Lander ES, Engreitz JM. Science. 2016 Sep 29. pii: aag2445. 10.1126/science.aag2445 PubMed 27708057