pYES2-HTH-HHT2
(Plasmid
#86482)
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Purposeyeast expression of HHT2 with His10-TEV-HA tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepYES2-HTH
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Backbone manufacturerAkash Gunjan, Addgene Plasmid 86470
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHHT2
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Alt nameHistone H3
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneHHT2 (a.k.a. YNL031C)
- Promoter GAL1
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Tag
/ Fusion Protein
- HIS10-TEV-HA (HTH) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GAL1
- 3′ sequencing primer CYC1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES2-HTH-HHT2 was a gift from Akash Gunjan (Addgene plasmid # 86482 ; http://n2t.net/addgene:86482 ; RRID:Addgene_86482) -
For your References section:
Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis. Singh RK, Kabbaj MH, Paik J, Gunjan A. Nat Cell Biol. 2009 Aug;11(8):925-33. doi: 10.1038/ncb1903. Epub 2009 Jul 5. 10.1038/ncb1903 PubMed 19578373
